In this pilot study, a comprehensive approach was undertaken to identify and quantify microplastic exposure among the participant group. The methodology encompassed two main components: environmental sampling and biological analysis. For environmental sampling, air and water samples were meticulously collected from locations frequented by the college students. Each sample was then subjected to filtration, using a fine mesh to capture microplastics, followed by subsequent visual inspection under a microscope to facilitate identification.

In parallel, biological samples—specifically fecal and urine specimens—were gathered to assess internal microplastic exposure. These samples underwent chemical characterization through Fourier-transform infrared spectroscopy (FTIR), enabling a detailed breakdown of microplastic types and concentrations. The integration of these techniques provided a comprehensive picture of microplastic sources and pathways. Data collection methods included:

  • Microscopic analysis for size and shape characterization
  • FTIR spectroscopy for chemical composition identification
  • Environmental monitoring tools to analyze abiotic factors influencing microplastic presence

To ensure reliability, all methodologies adhered to standardized protocols. A subset of samples underwent replicate analyses to establish a robust statistical framework. The study also utilized a survey questionnaire designed to capture behavioral and lifestyle patterns of the participants, including dietary habits and exposure to potential microplastic sources. This added a qualitative dimension to the quantitative data, enhancing the overall understanding of exposure levels. Below is a summary table highlighting the key techniques employed:

Technique Purpose Outcome
environmental Sampling Identify microplastics in air and water Quantitative analysis of external exposure
Microscopy Visual identification of microplastic characteristics Classification by size and shape
FTIR Spectroscopy Characterize chemical composition Identification of microplastic types
Survey Questionnaire Gather behavioral data Contextual understanding of exposure sources